Influence of Non-Thermal Atmospheric Pressure Plasma Jet on Extracellular Activity of α-Amylase in Aspergillus oryzae

In a previous study, we found that plasma can enhance spore germination and α-amylase secretion in A. oryzae, beneficial fungus used fermentation. To confirm this, the current investigated effects of on development using an enlarged sample size different source: jet. There was ~10% (p < 0.01) increase upon non-thermal atmospheric pressure jet (NTAPPJ) treatment for 5 min 10 min, as compared with control (no treatment). The activity detected potato dextrose broth (PDB) media during incubation significantly elevated plasma-treated samples, more obvious 15 treatments 24–96 h periods. levels oxidation reduction potential (ORP) NOX (nitrogen oxide species) were higher samples than suggesting these two variables could serve standard indicators enhancing after treatment. Genome sequencing analysis showed approximately 0.0016–0.0017% variations (changes 596–655 base pairs out total 37,912,014 pairs) genomic DNA sequence oryzae Our results suggest NATPPJ extracellular α-amylase, probably by increasing ORP to optimum level.